Robert Turesky, BSc, PhD

Professor, Department of Medicinal Chemistry
turesky

Contact

Office Phone
612-626-0141
Office Address

2-149 CCRB
Minneapolis, MN 55455
United States

Connect With Us

Titles

Professor, Department of Medicinal Chemistry
Masonic Chair in Cancer Causation

Education

PhD, Nutrition and Food Science, MIT

BSc, Biochemistry, University of Massachusetts

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Biography

Expertise

Metabolism, Biomarkers, Mass spectrometry

Awards & Recognition

  • Scientific Advisory Board of the United States Environmental Protection Agency from 2016 – 2017
  • Chosen as Distinguished Foreign Scientist, Cover Issue, “Novel aromatic amine mutagens, unintentionally formed from azo dyes, in river water in Kyoto” Japanese Journal of Cancer Research, 1998 Sep;89(9): Inside front cover. PubMed PMID: 9818020, 1998
  • B.Sc., Magna cum laude, in Biochemistry, University of Massachusetts, Amherst., 1978

Research Summary

Turesky Lab Group

The Turesky laboratory seeks to understand the roles of long-term exposures of environmental toxicants and endogenous electrophiles to causative factors for human diseases including cancer. We use human cell lines, primary cells, tissues and biological fluids to investigate the toxicity of carcinogens, characterize their metabolic pathways, and their ability to form DNA and protein adducts in humans. There are several chemicals present in the diet, tobacco smoke, and in traditional medicinal herbs that are the focus of our investigations. The laboratory employs state-of-the-art-mass spectrometry methods to conduct metabolic and biomarker studies in humans.

Heterocyclic aromatic amines (HAAs) are potential human carcinogens formed in cooked meat and some arise in tobacco smoke. The chemistry of HAA adduct formation with DNA and blood proteins is under study, to develop biomarkers for human cohorts designed to address the role of these chemicals in the diet and the risk of developing cancer. Our goal is to see if differences in dietary habits or metabolic processing of HAAs and their ensuing DNA and protein adducts, can be employed to better understand chemical exposures interindividual susceptibilities.

Aristolochic acids are nitrophenanthrene derivatives present in herbs of Aristolochia, some of which are still found in traditional herbal remedies. A long-sought analytical method employing archived biopsy samples fixed in formalin and paraffin embedded (FFPE) tissues was established for measuring DNA damage, by mass spectrometric methods, of renal cancer patients exposed to aristolochic acid, a potent urothelial carcinogen present in some traditional Chinese medicines. This analytical approach allows us to retrospectively screen DNA damage in FFPE tissues for which there is a clinical diagnosis of cancer. We are exploring the versatility of this DNA retrieval method by examination of FFPE samples for DNA adducts associated with hazardous chemicals in cigarette smoke, cooked meats, and air pollution.

DNAand protein adductomics. Novel scanning approaches, by ion trap mass and high resolution accurate mass spectrometry, are under development to screen human biospecimens for a variety of DNA and blood protein adducts formed with carcinogens present in the diet and environment. These screening methods are expected to provide us with a better understanding of the major chemical exposures that damage the genome and proteins, and lead to the onset of diseases including cancer.

Exfoliated urinary cells are being used to develop and characterize DNA adduct biomarkers that may help to explain the origin or urinary tract cancers in tobacco smokers.

Publications

PubMed