William Oetting, PhD

Professor, Department of Experimental and Clinical Pharmacology (ECP)

William Oetting

Contact Info

oetti001@umn.edu

Office Phone 612-624-1139

Fax 612-624-6645

Lab Phone 612-625-5628

Office Address:
B133 PWB

Mailing Address:
University of Minnesota
College of Pharmacy
Department of Experimental and Clinical Pharmacology
7-115 Weaver-Densford Hall
308 Harvard St. SE
Minneapolis, MN 55455

Biochemistry, University of California-Riverside

PhD, Genetics, University of Nebraska-Lincoln

Genetics, University of Nebraska-Lincoln

Microbiology, University of Kansas

Summary

The focus of my research is to identify genetic variants associated with complex human phenotypes and disease. My initial research was to identify mutations associated with defects in pigmentation. This research utilized linkage analysis, gene identification and DNA sequencing for mutation identification. In later work I used different analytical methods to identify alterations in proteins and metabolites associated with kidney transplant outcomes. I identified several urinary proteins to create a profile associated with acute rejection in kidney allografts. The goal of this work is to identify a set of biomarkers to determine disease status, especially in the sub-acute phase of organ rejection. Currently, my research is in the identification of genetic variation associated with various outcomes in kidney allograft recipients, including pharmacogenomic related outcomes. This work includes creating a large cohort of DNA samples from both kidney allograft recipients and living kidney donors, collecting clinical outcomes, genotyping variants and associating these variants with different phenotypes. Additional research in complex disease includes identification of variants associated with behavioral phenotypes, asthma, and novel single gene disorders. I am also working with whole exome sequencing (WES) and whole genome sequencing (WGS) to identify variants associated with single gene disorders and predisposing variants associated with glioblastoma.

Expertise

Genetics, Genomics

Awards & Recognition

  • The Vitiligo Research Award (Skin Disease Society, Inc.)
  • Young Investigator Award (PanAmerican Society for Pigment Cell Research)
  • Honorary Member, (PanAmerican Society for Pigment Cell Research)

Professional Associations

American Society of Human Genetics (ASHG)
American Society of Transplantation (AST) 
Human Genome Organization (HUGO) 
Human Genome Variation Society (HGVS) 
Human Variome Project (HVP) 
PanAmerican Society for Pigment Cell Research (PASPCR) 

Research

Research Summary/Interests

  • Genetic analysis of genes and gene polymorphisms associated with solid organ transplant outcome
  • Genetics of behavioral disorders
  • Proteomic and metabolomic analysis of kidney disease

Publications

  • Mohamed ME, Schladt DP, Guan W, Wu B, van Setten J, Keating BJ, Iklé D, Remmel RP, Dorr CR, Mannon RB, Matas AJ, Israni AK, Oetting WS, Jacobson PA; DeKAF Genomics and GEN03 Investigators. Tacrolimus troughs and genetic determinants of metabolism in kidney transplant recipients: A comparison of four ancestry groups. American Journal of Transplantation (in press), 2019. [PMID: 30801552]
  • Stapleton CP, Heinzel A, Guan W, van der Most PJ, van Setten J, Lord GM, Keating BJ, Israni AK, de Borst MH, Bakker SJL, Snieder H, Weale ME, Delaney F, Hernandez-Fuentes MP, Reindl-Schwaighofer R, Oberbauer R, Jacobson PA, Mark PB, Chapman FA, Phelan PJ, Kennedy C, Sexton D, Murray S, Jardine A, Traynor JP, McKnight AJ, Maxwell AP, Smyth LJ, Oetting WS, Matas AJ, Mannon RB, Schladt DP, Iklé DN, Cavalleri GL, Conlon PJ; UK Ireland Renal Transplant Consortium; DeKAF Genomics and GEN03 Studies; International Genetics and Translational Research in Transplantation Network. The impact of donor and recipient common clinical and genetic variation on estimated glomerular filtration rate in a European renal transplant population. American Journal of Transplantation (in press), 2019. [PMID: 30920136]
  • Oetting WS, Wu B, Schladt DP, Guan W, van Setten J, Keating BJ, Iklé D, Remmel RP, Dorr CR, Mannon RB, Matas AJ, Israni AK, Jacobson PA; DeKAF Genomics and GEN-03 Investigators. Genetic Variants associated with immunosuppressant pharmacokinetics and adverse effects in the DeKAF Genomics genome wide association studies. Transplantation 103:1131-1139, 2019. [PMID: 30801552]
  • Oetting WS, Schladt DP, Dorr CR, Wu B, Guan W, Remmel RP, Iklé D, Mannon RB, Matas AJ, Israni AK, Jacobson PA; DeKAF Genomics and GEN03 Investigators. Analysis of 75 candidate SNPs associated with acute rejection in kidney transplant recipients: Validation of rs2910164 in microRNA MIR146A. Transplantation (in press), 2019. [PMID: 30801535]
  • Dorr CR, Wu B, Remmel RP, Muthusamy A, Schladt DP, Abrahante JE, Guan W, Mannon RB, Matas AJ, Oetting WS, Jacobson PA, Israni AK; for DeKAF Genomics. Identification of genetic variants associated with tacrolimus metabolism in kidney transplant recipients by extreme phenotype sampling and next generation sequencing. Pharmacogenomics Journal (in press), 2018. [PMID: 30442921] [PMCID: PMC6522337]
  • Wu JF, Muthusamy A, Al-Ghalith GA, Knights D, Guo B, Wu B, Remmel RP, Schladt DP, Alegre M-L, Oetting WS, Jacobson PA Israni AK. Urinary microbiome associated with chronic allograft dysfunction in kidney transplant recipients. Clinical Transplantation e13436, 2018. [PMID: 30372560]
  • Seibert SR, Schladt DP, Wu B, Guan W, Dorr C, Remmel RP, Matas AJ, Mannon RB, Israni AK, Oetting WS, Jacobson PA. Tacrolimus trough and dose intra-patient variability and CYP3A5 genotype: Effects on acute rejection and graft failure in European American and African American kidney transplant recipients. Clinical Transplantation e13424, 2018. . [PMID: 30318646]
  • Scheibner A, Remmel R, Schladt D, Oetting WS, Guan W, Wu B, Dorr C, Israni A, Jacobson PA. Tacrolimus elimination in four subjects with a CYP3A5*3/*3 CYP3A4*22/*22 genotype. Pharmacotherapy, 38:e46-e52, 2018. [PMID: 29804290]
  • Snoek R, van Setten J, Keating BJ, Israni AK, Jacobson PA, Oetting WS, Matas AJ, Mannon RB, Zhang Z, Zhang W, Hao K, Murphy B, Reindl-Schwaighofer R, Heinzl A, Oberbauer R, Viklicky O, Conlon PJ, Stapleton CP, Bakker SJL, Snieder H, Peters EDJ, van der Zwaag B, Knoers NVAM, de Borst MH, van Eerde AM. NPHP1 (Nephrocystin-1) gene deletions cause adult-onset ESRD. Journal of the American Society of Nephrology, 29:1772-1779, 2018. [PMID: 29654215] [PMCID: PMC6054334]
  • Oetting WS, Wu B, Schladt DP, Guan W, Remmel RP, Dorr C, Mannon RB, Matas AJ, Israni AK, Jacobson PA for the DeKAF Investigators. Attempted Validation of 44 Reported SNPs Associated with Tacrolimus Troughs in a Cohort of Kidney Allograft Recipients. Pharmacogenomics 19:175-184, 2018. [PMID: 29318894] [PMCID: PMC6021962]
  • Oetting WS, Wu B, Schladt DP, Guan W, Remmel RP, Mannon RB, Matas AJ, Israni AK, Jacobson PA for the DeKAF Investigators. Genome wide association study identifies the common variants in CYP3A4 and CYP3A5 responsible for variation in tacrolimus trough concentration in Caucasian kidney transplant recipients. Pharmacogenomics Journal 18:501-505, 2018. [PMID: 29160300]
  • Dorr CR, Remmel RP, Muthusamy A, Fisher J, Moriarity BS, Yasuda K, Wu B, Guan W, Schuetz EG, Oetting WS, Jacobson PA, Israni AK. CRISPR/Cas9 genetic modification of CYP3A5 *3 in human hepatocytes leads to cell lines with increased midazolam and tacrolimus metabolism. Drug Metabolism and Disposition 45:957-965, 2017. [PMID: 28533324] [PMCID: PMC5518718]
  • Oetting WS, Dorr C, Remmel RP, Matas AJ, Israni AK, Jacobson PA. Concepts of genomics in kidney transplantation. Current Transplantation Reports 4:116-123, 2017. [PMID: 29123971] [PMCID: PMC5673265]
  • Sanghavi K, Brundage RC, Miller MB, Schladt DP, Israni AK, Guan W, Oetting WS, Mannon RB, Remmel RP, Matas AJ, Jacobson PA. Genotype-guided tacrolimus dosing in African American kidney transplant recipients. Pharmacogenomics Journal 17:61-68, 2017. [PMID: 26667830] [PMCID: PMC4909584]
  • Oetting WS, Jacobson PA, Israni AK. Validation is critical for GWAS-based associations. American Journal of Transplantation 17:318-319, 2017. [PMID: 27640398] [PMCID: PMC5266629]
  • Oetting WS, Schladt DP, Guan W, Miller MB, Remmel RP, Dorr C, Sanghavi K, Mannon RB, Herrera B, Matas AJ, Salomon DR, Kwok PY, Keating BJ, Israni AK, Jacobson PA for the DeKAF Investigators. Genome wide association study of tacrolimus concentrations in African American kidney transplant recipients identifies multiple CYP3A5 alleles. American Journal of Transplantation 16:574-582, 2016. [PMID: 26485092] [PMCID: PMC4733408]
  • Kalman LV, Agúndez JA, Appell ML, Black JL, Bell GC, Boukouvala S, Bruckner C, Bruford E, Bruckner C, Caudle K, Coulthard S, Daly AK, Del Tredici AL, den Dunnen JT, Drozda K, Everts R, Flockhart D, Freimuth R, Gaedigk A, Hachad H, Hartshorne T, Ingelman-Sundberg M, Klein TE, Lauschke VM, Maglott DR, McLeod HL, McMillin GA, Meyer UA, Müller DJ, Nickerson DA, Oetting WS, Pacanowski M, Pratt VM, Relling MV, Roberts A, Rubinstein WS, Sangkuhl K, Schwab M, Scott SA, Sim SC, Thirumaran RK, Toji LH, Tyndale R, van Schaik RH, Whirl-Carrillo M, Yeo KJ, Zanger UM. Pharmacogenetic allele nomenclature: International workgroup recommendations for test result reporting. Clinical Pharmacology & Therapeutics 99:172-185, 2016. [PMID: 26479518] [PMCID: PMC4724253]

Teaching

Teaching Areas

Human Genetics, Molecular Biology

Courses

  • BIOL 1101 Genetics and Society
  • GCD 4143 Human Genetics 
  • PHAR 6702 Integrated Biochemical Sciences 
  • PHAR 6224 Pharmacogenomics