Byeong Hwa Yun, PhD

Research Assistant Professor, Department of Medicinal Chemistry

Byeong Hwa Yun

Contact Info

bhyun@umn.edu

Office Phone 612-625-2643

Lab Phone 612-651-2302

Office Address:
2231 6th Street SE, CCRB 2-122, Minneapolis, MN 55455

Lab Address:
2231 6th Street SE, CCRB 2-240 B92, Minneapolis, MN 55455

Additional Locations

Masonic Cancer Center

Research Assistant Professor, Department of Medicinal Chemistry


PhD, Chemistry, New York University, New York

MS, Biophysical Chemistry, Yeungnam University, South Korea

Summary

My research focuses on biomonitoring of carcinogen-DNA adducts in humans derived from environmental and endogenous genotoxicants. Employing advanced techniques of LC-mass spectrometry, I established methods to measure DNA adducts of aristolochic acids (AA), a renal carcinogen found in herbal plant Aristolochia, at a trace level employing freshly frozen and formalin-fixed paraffin embedded (FFPE) human tissues. The FFPE tissue block is the most common method to preserve tissues obtained from biopsy, however, it has not been utilized for DNA biomarker discovery due to technical difficulties. Our methods of DNA retrieval from FFPE tissues can fully reverse the crosslinks in the tissues under a mild condition and preserve the DNA adducts during the tissue process. The development of the DNA retrieval method for FFPE tissue is a major technological advancement in DNA adduct biomarker research. Recently, the method of DNA retrieval from FFPE has been extended for DNA adducts of multiple classes of carcinogens including aristolochic acids (AA), heterocyclic aromatic amine (HAAs), aromatic amine, polycyclic aromatic hydrocarbons (PAHs), and tobacco carcinogens. The methodology is now primed to examine various adducts in human cohorts where fresh frozen tissue samples are not available.

Expertise

nanoESI-UHPLC-mass spectrometry, analysis of DNA damage by photo-oxidation, laser photolysis, polarized spectroscopy (Circular/Linear Dichroism), Flourescence spectroscopu 

Professional Associations

American Chemical Society

Languages

  • English
  • Korean

Research

Research Summary/Interests

Biochemistry & Biophysics

Publications

1. Yun, B. H., J. Guo, M. Bellamri, and R. J. Turesky. "DNA adducts: Formation, biological effects, and new biospecimens for mass spectrometric measurements in humans." Mass Spectrometry Reviews 2018, 1-28, Impact factor (2017), 9.526

2. Yun, B.H., Bellamri, M., Krishnamachari, S., Turesky, R.J., Devised Model System to Measure Carcinogen-DNA Adducts in Exfoliated Urinary Cells from Bladder Cancer Patients Employing Human Bladder Cancer Cell line. Analytical Chemistry 2018 (Online published) Impact factor (2017), 6.042, DOI: 10.1021/acs.analchem.8b02170
3. Yun, B. H., J. Guo and R. J. Turesky. "Formalin-Fixed Paraffin-Embedded Tissues—An Untapped Biospecimen for Biomonitoring DNA Adducts by Mass Spectrometry." Toxics 2018, 6 (30). (Cover Article)
4. Springer, S. U.; Chen, C.-H.; Pena, M. D. C. R.; Li, L.; Douville, C.; Wang, Y.; Cohen, J. D.; Taheri, D.; Silliman, N.; Schaeffer, J.; Ptak, J.; Dobbyn, L.; Papoli, M.; Kinde, I.; Afsari, B.; Tregnago, A. C.; Bezerra, S. M.; VandenBussche, C.; Fujita, K.; Ertoy, D.; Cunha, I. W.; Yu, L.; Bivalacqua, T. J.; Grollman, A. P.; Dickman, K. G.; Diaz, L. A.; Karchin, R.; Ludmila, D.; Huang, C.-Y.; Shun, C.-T.; Turesky, R. J.; Yun, B. H.; Rosenquist, T. A.; Pu, Y.-S.; Hruban, R. H.; Tomasetti, C.; Papadopoulos, N.; Kinzler, K. W.; Vogelstein, B.; Dickman, K. G.; Netto, G. J., Non-invasive detection of bladder cancer through the analysis of driver gene mutations and aneuploidy. eLife 2017. Impact factor (2017), 7.616
5. Yun, B. H., Xiao, S., Yao, L., Krishnamachari, S., Rosenquist, T. A., Grollman, A. P., Murugan, P., Weight, C. J., and Turesky, R. J. A Rapid Throughput Method to Extract DNA from Formalin-Fixed Paraffin-Embedded Tissues for Biomonitoring Carcinogenic DNA Adducts, Chemical Research in Toxicology, 2017 (e-published on November 9, 2017), DOI:10.1021/acs.chemrestox.7b00218, Impact factor (2017), 3.432
6. Xiao, S., Guo, J., Yun, B. H., Villalta, P. W., Krishna, S., Tejpaul, R., Murugan, P., Weight, C. J., Turesky, R. J., Biomonitoring DNA Adducts of Cooked Meat Carcinogens in Human Prostate by Nano Liquid Chromatography-High Resolution Tandem Mass Spectrometry: Identification of 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine DNA Adduct. Analytical Chemistry 2016, 88 (24), 12508-12515. DOI:10.1021/acs.analchem.6b04157, Impact factor (2017), 6.042
7. Hoang, M. L., Chen, C. H., Chen, P. C., Roberts, N. J., Dickman, K. G., Yun, B. H., Turesky, R. J., Pu, Y. S., Vogelstein, B., Papadopoulos, N., Grollman, A. P., Kinzler, K. W., Rosenquist, T. A., Aristolochic Acid in the Etiology of Renal Cell Carcinoma. Cancer epidemiology, biomarkers & prevention 2016, 25 (12), 1600-1608. DOI:10.1158/1055-9965.EPI-16-0219 , Impact factor (2017), 4.554

Full list of publications
https://www.ncbi.nlm.nih.gov/myncbi/browse/collection/48017510/?sort=date&direction=ascending