Suresh Pujari

Research Assistant Professor, Department of Medicinal Chemistry

Suresh Pujari

Contact Info

spujari@umn.edu

Office Phone 612-624-0638

Lab Phone 612-625-4936

Office Address:
CCRB 2-161
2231 6th Street SE
Minneapolis, MN 55455

Mailing Address:
2231 6th Street SE
CCRB 2-161
Minneapolis, MN 55455

Research Assistant Professor, Department of Medicinal Chemistry


PhD, Chemistry, University of Osnabrueck, Osnabrueck, Germany

MS, Organic Chemistry, Karnatak University Dharwad, India

Summary

My research is focused on design and synthesis of modified nucleic acids, amino acids and site-specific modified proteins.

DNA-Protein Cross-links: Living cells upon exposure to different sources like ionizing radiation, UV irradiation, ROS and other endogenous and exogenous agents lead to covalent trapping of cellular proteins on genomic DNA resulting in super bulky lesions called DNA-Protein Cross-links (DPCs). These lesions have consequential effect on cellular transactions like transcription, replication and DNA repair. Research in our group focus on development of new methods to synthesize these lesions and study their effects on cells and regulation of gene expression.

Formamidopyrimidines: DNA in all living organisms undergoes a several types of damages, which are believed to play pivotal role in various diseases like cancer. The damage can be base alterations, DNA strand breaks and inter or intra strand cross-links. Our lab is currently working on design, synthesis and biological evaluation of formamidopyrimidines (FAPy) of adenine and guanine – nucleobase altered lesions resulting upon rupture of 5-membered ring. We are focused on the FAPy lesions of epoxybutene and diepoxybutane which are the reactive metabolites of 1,3-butadiene, a known carcinogen.

Expertise

Organic/Bioorganic Chemistry and Chemical Biology

Research

Publications

PubMed

  • Pujari, S. S., Zhang, Y., Ji, S., Distefano, M., Tretyakova, N. Site-specific cross-linking of proteins to DNA via a new bioorthogonal approach employing oxime ligation. Chemical Communications, 2018, 54, 6296-6299.
  • D. Kotandeniya; C. L. Seiler; J. Fernandez; Pujari, S. S., L. Curwick; K. Murphy; S. Wickramaratne; S. Yan; D. Murphy; Y. Y. Sham and N. T. Tretyakova. Can 5-methylcytosine analogues with extended alkyl side chains guide DNA methylation? Chem. Commun. 2018, 54, 1061-1064.
  • Pujari S. S., Tretyakova N. Chemical Biology of N5-Substituted Formamidopyrimidine DNA Adducts. Chem Res Toxicol. 2017 30, 434-452.
  • Pujari, S. S.,Ingale, S. A., Seela, F* High-Density Functionalization and Cross-linking of DNA: “Click” and “Bis-Click” Cycloadditions Performed on Alkynylated Oligonucleotides with Fluorogenic Anthracene Azides. Bioconjugate Chem.2014,21, 1629–1641.
  • Pujari, S. S.,Seela, F* Oligonucleotides with "Clickable" Sugar Residues: Synthesis, Duplex Stability, and Terminal versus Central Inter-Strand Cross-Linking of 2'-O-Propargylated 2-Aminoadenosine with a Bifunctional Azide.J. Org. Chem.2014,79, 4423-4437.
  • Pujari, S. S., Seela, F* ParallelStranded DNA Stabilized with Internal Sugar Crosslinks: Synthesis and Click Ligation of Oligonucleotides Containing 2’-Propargylated Isoguanosine.J. Org. Chem.2013, 78, 8545-8561.