The Clinical Pharmacology Analytical Services laboratory (CPAS) resides in the Department of Experimental and Clinical Pharmacology (ECP) in the College of Pharmacy.
CPAS offers quantification services of drug concentrations in biological samples. The CLIA-certified lab and its team of experts provide research and clinicians the resources to measure drug concentrations in blood and tissue samples of human and animal origin, including animals as patients and animals serving as research models. The lab which opened in October 2003 uses state-of-the-art equipment to facilitate analysis of drugs, xenobiotics, endogenous compounds and biomarkers in tissue samples. Capabilities of the core lab include HPLC with UV, fluorescent or electrochemical detection, LC-MS, LC-MS/MS and GC-MS. Additional, UV and fluorescent plate readers and liquid scintillation counters are available for conducting ELISA, RIA and other assays in a 96-well plate format. Because of its flexible structure, customers can purchase only the services they need, including assay consultation, assay development and validation, conduct of assays and application. Lab services are made available to researchers from within and outside the University of Minnesota community.
Also housed within ECP is the Center for Forecasting Drug Response. The coordination between the lab and the center provides unique opportunities for further study of relationships between drug concentrations and patient response.
Services provided by CPAS include:
- Develop and validate methods for sample analysis
- Measure concentrations of the compound of interest in samples
- Isolate, purify, and conduct structural determination of novel compounds, metabolites and biomarkers
Beyond reporting of drug concentrations to the investigator or clinician, additional services include:
- Traditional pharmacokinetic/pharmacodynamic analyses
- Design of future clinical trials based on pharmacokinetic/pharmacodynamic data
- Development and analysis of limited sampling designs for large clinical trials or trials in which extensive sampling is not possible
- Population pharmacokinetic analysis of the data
- Assistance in grant applications
The Core Lab is particularly adept at analyses of small molecules, though analysis of peptides and antibodies are available on a case-by-case basis. Among the list of most commonly analyzed compounds are:
Antineoplastics
- Cyclophosphamide and the active metabolite, 4-hydroxycyclophosphamide
- F-ara-A, the biologicall relevant metabolite of fludarabine
- Gemcitabine
Cytochrome P450 phenotyping
- Dextromethorphan/dextrorphan for CYP2D6
- Caffeine and metabolites CYP1A2
- Dextromethorphan and metabolites CYP2D6 and CYP3A
- Flubiprofen and metabolite for CYP2C9
- Chlorzoxazone and metabolite for CYP2E1
Miscellaneous
- Mycophenolic acid and mycophenolic acid glucuronide (total and unbound)
- Non-steroirdal anti-inflammatory agents
- Benzodiazepines
- Fenofibric acid and fenofibric acid glucuronide
- Metoprolol,R- and S- enantiomers
Antiepileptics
- Valproic acid carbamazepine and metabolites
- Phenytoin, R- and S- enantiomers
- Fosphenytoin, R- and S-enantiomers
Hormones
- Epinephrine
- Norepinephrine
- Cortisol
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